Development and Validation of New RP-HPLC Method For Simultaneous Estimation of Gatifloxacin and Difluprednate In Pharmaceutical Dosage Forms
نویسنده
چکیده
A simple, selective, precise and accurate Reverse Phase High Pressure LiquidChromatographic (RPHPLC) method was developed and validated for the simultaneousestimation ofgatifloxacin and difluprednate in pharmaceutical dosage forms.The method was validated for accuracy, specificity, linearity and robustness asper the ICH guidelines.Binary elution at a flow rate of 1.0 mL per min was employed onEnable C18column at temperature of 25C. The method was successfully applied to estimategatifloxacin and difluprednate in marketed ophthalmic dosage form since there was nointerference from the excipients.
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